In the new 3rd revised and updated edition, all the chapters have been modified with the latest advances in this field. Another aspect of this edition is that the most commonly encountered artifacts have been covered in each stage of the technique. This will tremendously help the persons in their day-to-day work flow. Troubleshooting in hematoxylin and eosin staining is another area which has been covered in detail. The new edition brings forth two new Chapters; one on Immunofluorescence Technique and the other on Cytopathology Technique which is a part and partial of small and medium sized laboratories. Colored photographs have been inserted wherever relevant in each chapter. The colored photographs in the chapter “special stains”, will help students to understand how the positive result looks under a microscope. Ten viva questions each on most common special staining techniques are included for the benefit of students/technologists. By answering these questions, they will understand the techniques and its importance better. In the chapter immunohistochemistry, the subject is updated along with the addition of automation. A comparative table of different models of machine is also included to understand the basic difference between them. Quality aspect is included in the chapter Organization of Histology Laboratory. This would immensely help the technologist to improve the standard of their laboratory